(B) Superoxide detection in


Superoxide dismutases (SODs) are metalloproteins that protect organisms from toxic reactive oxygen species by catalyzing the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. Chlorovirus PBCV-1 encodes a 187-amino-acid protein that resembles a Cu-Zn SOD with all of the conserved amino acid residues for binding copper and zinc (named cvSOD). cvSOD has an internal Met that results in a 165-amino-acid protein (named tcvSOD). Both cvSOD and tcvSOD recombinant proteins inhibited nitroblue tetrazolium reduction of superoxide anion generated in a xanthine-xanthine oxidase system in solution. tcvSOD was chosen for further characterization because it was easier to produce. Recombinant tcvSOD also inhibited a riboflavin photochemical reduction system in a polyacrylamide gel assay, which was blocked by the Cu-Zn SOD inhibitor cyanide but not by azide, which inhibits Fe and Mn SODs. A kcat/Km value for cvSOD was determined by stop-flow spectrophotometry as 1.28 X 108 M-1 S-1, suggesting that cvSOD-catalyzed O2- dismutation was not a diffusion controlled encounter. The cvsod gene was expressed as a late gene, and cvSOD activity was detected in purified virions. Superoxide accumulated rapidly during virus infection, and circumstantial evidence indicates that cvSOD aids its decomposition to benefit virus replication. Cu-Zn SOD homologs have been described to occur in 3 other families of large DNA viruses, poxviruses, baculoviruses, and mimiviruses, which group as a clade. Interestingly, cvSOD does not group in the same clade as the other virus SODs but instead groups in an expanded clade that includes Cu-Zn SODs from many cellular organisms.

Read electro-mechanical engineering blog
You might also like
Biogenesis - Sono File (Superoxide 2nd rmx)
Biogenesis - Sono File (Superoxide 2nd rmx)
Genetic polymorphism and catharsis of superoxide dismutase
Genetic polymorphism and catharsis of superoxide dismutase
Superoxides and Superoxide Dismutase: Physiology
Superoxides and Superoxide Dismutase: Physiology ...
Metal New Metal Polish - 4 oz bottle - Safely Remove Years of Tarnish and Oxidation From Sterling Silver, Gold or Silver Jewelry, Uncoated Aluminum, Brass, Copper, & Stainless Steel - Quickly Restore the Original Brilliant Shine - Ammonia Free - Made in USA - 100% Satisfaction Guaranteed
Health and Beauty
  • SHINES ANY UNCOATED METAL! Quickly remove oxidation and restore a BRILLIANT shine to dulled metals.
  • Simply use a dime-size amount on a soft cloth or applicator pad and rub until it begins to turn black. Then polish with another clean cloth until you see the desired...
  • Metal New is AMONIA-FREE so it doesn t have the intense, harsh smell that you may have experienced with some other brands. Metal New is VOC COMPLIANT.
sunnyshopday sunnyshopdayTibetan10020 Tibetan Silve hand chain bracelet Bangle jewelry sterling silver quality jewel
Jewelry (sunnyshopday)
  • find jewel sets in affordable ,search sunnyshopdayset1
  • do not wear it when bath or wash,this jewel contain silver, oxidation reaction can make the jewel change color when touch water.
  • nice hand chain.bracelet,do not miss it,It is nice jewel ,good choice for gift or birthday and ChristmasIt is nice jewel ,good choice for gift or birthday and Christmas
Update on the Hawaiian Raw Superoxide Dismutase
Update on the Hawaiian Raw Superoxide Dismutase
Superoxide - Square Purity
Superoxide - Square Purity
Klasky Crappy Csudo Classy Suppose Superoxide.wut
Klasky Crappy Csudo Classy Suppose Superoxide.wut
sntusa001 Sinuote 6pcs Oxidation Silver Archery Arrows 2-Blade Expandible Broadhead Tip 2" Cut
Sports (sntusa001)
  • The solid steel ferrule sports a brand new hybrid tip design
  • Allowing the Hypodermic to have the aerodynamics and accuracy of a leading edge blade, coupled with the bone crushing performance of our chisel tip.
  • Material:Aluminum & 350 Stainless Steel
  • Fixed blade with Two Stainless Steel Blades
  • Included in the Package:6X pcs
MusicNomad Equipment Care Music Nomad Silver Polish for Silver & Silver Plated Instruments 4oz. Bottle
Musical Instruments (MusicNomad Equipment Care)
  • Cleans & Polishes
  • Removes Tarnish & Oxidation
  • Residue & Streak Free
  • Anti-Tarnish Formulation for Extra Protection
  • Smells Great!
PMC Supplies LLC 6 oz Pickle Pickling Powder Deoxidizing Flux Cleaning Compound For Gold Silver Copper Brass Jewelry Cleaner
Art and Craft Supply (PMC Supplies LLC)
  • Great for pickling jewelry and metal components and removing surface oxidatio
  • Deoxidizing Cleaning Compound
  • Non-flammable and non-explosive
  • Cleans Gold, Silver, Copper, Brass
  • Made In The USA
Related Posts