Free nitric oxide samples
Nitric oxide (NO) is a multifunctional cell signaling molecule produced through the action of nitric oxide synthase (NOS). Cayman’s Nitric Oxide Fluorometric Cell-Based Assay Kit detects NO in living cells by using fluorescein amine methyl ester (FA-OMe). When the non-fluorescent FA-OMe senses free NO in living cells it forms an intensely fluorescent deamination product, dA-FA-OMe whose fluorescence is directly proportional to the NO being produced by cells. dA-FA-OMe fluorescence can also be used to indirectly examine the NOS activity responsible for NO production. The NOS inhibitor, L-NAME, is also included for this purpose. This assay can be used to directly examine the effect of drugs, cytokines, or other experimental agents on NO production in cultured cells.
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Shiue, T., Chen, Y., Wu, C., et al. Nitric oxide turn-on fluorescent probe based on deamination of aromatic primary monoamines. Inorg Chem 51 (2012).Show all 5 Hide all but first 3
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