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June 27, 2015 – 07:21 am

Nitric Oxide Cell-Based Assay Kit

Cayman Chemical Item Number 10009419

Description

Nitric oxide (NO) is a multifunctional cell signaling molecule produced through the action of nitric oxide synthase (NOS). Cayman’s Nitric Oxide Fluorometric Cell-Based Assay Kit detects NO in living cells by using fluorescein amine methyl ester (FA-OMe). When the non-fluorescent FA-OMe senses free NO in living cells it forms an intensely fluorescent deamination product, dA-FA-OMe whose fluorescence is directly proportional to the NO being produced by cells. dA-FA-OMe fluorescence can also be used to indirectly examine the NOS activity responsible for NO production. The NOS inhibitor, L-NAME, is also included for this purpose. This assay can be used to directly examine the effect of drugs, cytokines, or other experimental agents on NO production in cultured cells.

Synonyms Stability 1 year Storage -20°C Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Bredt, D.S., and Snyder, S.H. Nitric oxide: A physiologic messenger molecule. Annu Rev Biochem 63 (1994).

Garcin, E.D., Arvai, A.S., Rosenfeld, R.J., et al. Anchored plasticity opens doors for selective inhibitor design in nitric oxide synthase. Nat Chem Biol 4(11) (2008).

Allen, J.B., Keng, T., and Privalle, C. Nitric oxide and peroxynitirite production in ocular inflammation. Environ Health Perspect 106 (1998).

Nathan, C. Nitric oxide as a secretory product of mammalian cells. FASEB J 6 (1992).

Shiue, T., Chen, Y., Wu, C., et al. Nitric oxide turn-on fluorescent probe based on deamination of aromatic primary monoamines. Inorg Chem 51 (2012).

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Source: www.caymanchem.com

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