Nitrous oxide reductase

Cover imageTwo PCR primer sets for the nitrous oxide reductase gene (nosZ) were developed. The initial primers were based on three sequences in GenBank and used to amplify nosZ from continental shelf sediments and from two denitrifiers in culture, Thiosphaera pantotropha and Pseudomonas denitrificans. Three unique marine sediment nosZ genes were identified and sequenced. The marine nosZ genes were most closely related to the nosZ genes of Paracoccus denitrificans or to Rhizobium meliloti. Alignment of all nosZ sequences currently available (n=10) facilitated redesign of the PCR primers. Three new primer sets which amplify 1100 bp, 900 bp and 250 bp regions of the nosZ gene were designed and tested. The new primers robustly amplified nosZ fragments from samples in which the initial nosZ primers were only marginally successful.

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MyBioSource Recombinant Pseudomonas stutzeri Nitric oxide reductase subunit B (norB) (Recombinant Protein)
BISS (MyBioSource)
  • GeneName: norB; norB
  • SynName: Recombinant Nitric oxide reductase subunit B (norB)
  • Nitric oxide reductase subunit B EC= 1.7.2.5
  • NOR large subunit Nitric oxide reductase cytochrome b subunit ; Nitric oxide reductase subunit B
  • NOR large subunit; Nitric oxide reductase cytochrome b subunit
  • Source: E Coli or Yeast
  • Purity: 90%; *Tag Information: His tagged
  • SeqPos: 2-474
  • Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
  • FREE-8GB-USBDrive for this item. Item is for research use. NOT for diagnostic/therapeutic procedure.
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